How Much Bacteriostatic Water to Mix with 5mg of Semaglutide (Complete Guide for Safe Reconstitution)
The Complete Visual Guide to Reconstituting Semaglutide
An easy-to-follow infographic for safely and accurately mixing a 5mg vial.
Why Precision Matters
99%
Of a peptide’s effectiveness relies on accurate reconstitution. Improper mixing can degrade the fragile molecules, leading to inaccurate dosing and reduced potency.
The Right Tool for the Job: Choosing Your Solvent
For multi-dose peptides like semaglutide, the choice of solvent is critical for safety and sterility over time.
Bacteriostatic (BAC) Water
The Recommended Standard
- ✔Contains a Preservative: Includes 0.9% benzyl alcohol, which prevents bacterial growth after the vial is opened.
- ✔Ideal for Multi-Dose Vials: Keeps the solution sterile for up to 28 days, ensuring every dose is safe.
- ✔Ensures Safety & Potency: The gold standard for reconstituting peptides intended for multiple administrations.
Sterile Water
For Single Use Only
100%
Pure H₂O
- ✖No Preservative: Lacks any antimicrobial agent, making it susceptible to contamination after the first puncture.
- ✖Single-Use Only: Once opened, any remaining water should be discarded to avoid bacterial growth.
- ✖Not for Multi-Dose Vials: Using it for multiple doses creates a significant risk of injection site infections.
Step-by-Step Reconstitution Guide
Follow this six-step process for a perfect, gentle mix every time. This ensures the peptide’s structure remains intact and your solution is sterile.
Prepare Workspace & Vials
Wash hands and disinfect your work surface. Wipe the rubber stoppers of both vials with alcohol pads.
Draw 2 mL of BAC Water
Use a sterile mixing syringe to carefully draw exactly 2 mL of bacteriostatic water.
Introduce Water Gently
Slowly inject the BAC water into the semaglutide vial, aiming the stream against the inside glass wall, not directly onto the powder.
Mix by Rolling, Not Shaking
Gently roll the vial between your fingers until all the powder has dissolved. Shaking will destroy the peptide!
Final Check for Clarity
The final solution must be completely clear. If it’s cloudy or has particles, discard it.
Refrigerate Immediately
Store the reconstituted vial in the refrigerator between 2°C and 8°C (36°F – 46°F), protected from light.
The Dosing Formula: 5mg Semaglutide + 2mL Water
This mixing ratio creates a simple concentration that makes calculating your dose straightforward using a standard U-100 insulin syringe.
With this concentration, every 1 unit on your insulin syringe is equal to 25mcg (0.025mg) of semaglutide.
Critical Do’s & Don’ts
Avoid common mistakes to ensure maximum safety and peptide potency.
Do 👍
- ✔Use high-quality Bacteriostatic Water for multi-dose vials.
- ✔Always roll or swirl the vial gently to mix.
- ✔Wipe both vial stoppers with alcohol before every use.
- ✔Store reconstituted solution in the refrigerator immediately.
Don’t 👎
- ✖NEVER shake the vial, as this will destroy the peptide.
- ✖Don’t inject water directly onto the powder cake.
- ✖Never use a solution that is cloudy or discolored.
- ✖Don’t use tap water, bottled water, or any non-sterile liquid.